The movie once again focuses on Ben Gates. For Ben Gates (Nicolas Cage), history isn’t just a dry record of what’s occurred in a distant and half-forgotten past…for him, history is alive, vital, and occasionally it offers the possibility of finding extraordinary treasures. After his astonishing discovery of the riches of the Templar Knights, Ben has become the world’s most famous treasure hunter…although he prefers the term “treasure protector.” Ben and his father, university professor Patrick Gates (Jon Voight), are shaken by the discovery of one of the long-lost pages from the diary of Lincoln assassin John Wilkes Booth. The diary was found on Booth’s body when he was killed; however, several pages had been torn from the diary and have never been found…until now. Surprisingly, the information on this page seems to implicate their ancestor Thomas Gates as a co-conspirator in the assassination of President Lincoln.

Ben must work with his now ex-girlfriend, American history archivist Abigail Chase (Diane Kruger), and his tech-wiz partner, Riley Poole (Justin Bartha), in a globe-trotting adventure which dangerously criss-crosses the inner sanctums of Washington, D.C., Paris, London and the American heartland.